My Honours Project 03

Our media for pathogens finally arrived!! Went to prepare the media yesterday so that me and Joce can do some sampling today. According to the protocol from my professor, I have to prepare phosphate saline buffer (PSB) with 3% NaCl for pre- enrichment and alkaline saline peptone water (APW) for enrichment of Vibrio. After a short meeting with him, he found out that he made a mistake and both pre- enrichment and enrichment should be done in APW with 3% NaCl.

Thinking that I can go back to the laboratory to add in the NaCl to the APW, I found out that I was too late as my PI had already put the solutions into the autoclave. So the whole of last night, I was worrying whether the lack of 3% NaCl will affect the enrichment process, or whether I can add in the NaCl and autoclave a second time. In the end, I choose not to add in the NaCl...

After we finish our laboratory for the day, I received a call from my professor...


Professor: YunLe?


Lele: Yes?


Professor: Have you prepared the APW?


Lele: Yup... *think: should I tell him I didn't add in the NaCl?


Professor: You prepared the APW and added to your sample already?


Lele: Yar... but I forget to add in the NaCl...


Professor: Oh, cause from the protocol of APW, the NaCl was already in the powder. So don't have to add NaCl...


Lele: So don't need to add NaCl? *Phew


Professor: You didn't add the NaCl?


Lele: Nope.


Professor: Hahaha, okay okay. Don't need to add...


Lele: Haha, okay.





---oOOo---

Wee-----!! Super lucky! I think my professor was happy cause I didn't wasted 40g of the powder. Lalala~





Le~