Our media for pathogens finally arrived!! Went to prepare the media yesterday so that me and Joce can do some sampling today. According to the protocol from my professor, I have to prepare phosphate saline buffer (PSB) with 3% NaCl for pre- enrichment and alkaline saline peptone water (APW) for enrichment of Vibrio. After a short meeting with him, he found out that he made a mistake and both pre- enrichment and enrichment should be done in APW with 3% NaCl.
Thinking that I can go back to the laboratory to add in the NaCl to the APW, I found out that I was too late as my PI had already put the solutions into the autoclave. So the whole of last night, I was worrying whether the lack of 3% NaCl will affect the enrichment process, or whether I can add in the NaCl and autoclave a second time. In the end, I choose not to add in the NaCl...
After we finish our laboratory for the day, I received a call from my professor...
Professor:
YunLe?
Lele: Yes?
Professor: Have you prepared the
APW?
Lele: Yup... *think: should I tell him I didn't add in the
NaCl?
Professor: You prepared the
APW and added to your sample already?
Lele:
Yar... but I forget to add in the
NaCl...
Professor: Oh, cause from the protocol of
APW, the
NaCl was already in the powder. So don't have to add
NaCl...
Lele: So don't need to add
NaCl? *Phew
Professor: You didn't add the
NaCl?
Lele: Nope.
Professor:
Hahaha, okay okay. Don't need to add...
Lele:
Haha, okay.
---oOOo---
Wee-----!! Super lucky! I think my professor was happy cause I didn't wasted 40g of the powder. Lalala~
Le~
